RNA

Part:BBa_K3001018:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lbu crRNA targeting RNA Mango


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 35
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 35
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 35
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 35
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The target sequence needs to be specific for just what you are targeting to ensure the effect you are seeing is because of the presence of the target. A direct repeat stem loop is also present to enable the crRNA to interact with Cas13a Lbu.


Source

Designed by Laura Keffer-Wilkes based on designs provided by Munich 2017. Synthesized by IDT.

References